[HTML][HTML] Membranous and structural damage that occur during cryopreservation of human sperm may be time-related events
P Desrosiers, C Légaré, P Leclerc, R Sullivan - Fertility and sterility, 2006 - Elsevier
OBJECTIVE: To evaluate sperm cryopreservation-induced injuries using sperm plasma
membrane protein P34H and α-tubulin as two different subcellular compartment markers.
DESIGN: Prospective experimental study. SETTING: Academic hospital research center and
fertility clinic. PATIENT (S): Semen samples obtained from healthy donors attending the
fertility clinic. Sperm samples were either directly processed for sodium dodecyl sulfate–
polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot experiments (control …
membrane protein P34H and α-tubulin as two different subcellular compartment markers.
DESIGN: Prospective experimental study. SETTING: Academic hospital research center and
fertility clinic. PATIENT (S): Semen samples obtained from healthy donors attending the
fertility clinic. Sperm samples were either directly processed for sodium dodecyl sulfate–
polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot experiments (control …
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